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lc3b staining  (MedChemExpress)


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    MedChemExpress lc3b staining
    Lc3b Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc3b+staining/LC3A%2FB+Antibody/pm42037167-276-17-30
    Average 95 stars, based on 33 article reviews
    lc3b staining - by Bioz Stars, 2026-09
    95/100 stars

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    Staining:

    Article Title: Targeting Endogenous Lipophagy: A Novel Strategy to Enhance MSC Osteogenesis and Mineralization for Senile Osteoporosis Therapy.
    Article Snippet: .. After cell adhesion, the cells were fixed with 4% PFA (neutral buffer, methanol-free) for 20 min. For LC3B staining, the cells were treated with chloroquine (100 μM for 3 h; MCE, Cat. HY-17589A) prior to fixation and staining. .. After fixation, the cells were washed three times with 1 × phosphate-buffered saline (PBS), permeabilized with 0.5% Triton X-100 for 20 min, and then blocked with PBS containing 10% fetal bovine serum (FBS) for 1 h. Following overnight incubation with the primary antibody against LC3B (Abmart, Cat. T55992S) or LAMP(Abcam, Cat.EPR21026) at 4◦C, the samples were incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor 555) (Abcam, Cat. ab150078) at room temperature for 1 h. Subsequently, the cells were stained with a 10 μmol ⋅L− 1 BODIPY 493/503 solution (MCE, Cat.HY-W090090 ) at room temperature for 1 h to label lipid droplets, followed by three washes with 1 × PBS.



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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) <t>Fluorescence</t> imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.
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    Image Search Results


    Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) Fluorescence imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.

    Journal: Materials Today Bio

    Article Title: Macrophage targeted cellular microparticles synergize anti-tuberculosis therapy combining CircRNA-mediated host immune defense and antibiotic killing

    doi: 10.1016/j.mtbio.2025.102489

    Figure Lengend Snippet: Gal-CircRNA@Rif@MPs promote autophagy of host cells in the lung of H37Ra infected mice. (A) Fluorescence imaging of DAPI, LC3B and LAMP1 staining in the lung of mice in different groups at the endpoint of experiment, scale bar: 25 μm. (B) Statistical analysis of the relative fluorescence intensity of LC3B in the lung of mice in different groups at the endpoint of experiment, n = 8, ***p < 0.001. (C) Statistical analysis of the relative fluorescence intensity of LAMP-1 in the lung of mice in different groups at the endpoint of experiment. n = 8, ***p < 0.001. (D) Typical TEM images for the lung tissues of mice with or without Gal-CircRNA@Rif@MPs treatment, scale bar: 1 μm.

    Article Snippet: Fluorescence staining of DAPI, LC3B (Cell Signaling 43566, USA) and LAMP1 (Cell Signaling 73294, USA) was used to determine autophagy in lung tissues.

    Techniques: Infection, Fluorescence, Imaging, Staining